Article
Recombinational inactivation of the gene encoding nitrate reductase in Aspergillus parasiticus.
Applied and environmental microbiology - 1 Sept 1993
Wu T S, Linz J E
Abstract excerpt
Functional disruption of the gene encoding nitrate reductase (niaD) in Aspergillus parasiticus was conducted by two strategies, one-step gene replacement and the integrative disruption. Plasmid pPN-1, in which an internal DNA fragment of the niaD gene was replaced by a functional gene encoding orotidine monophosphate decarboxylase (pyrG), was constructed. Plasmid pPN-1 was introduced in linear form into A....
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