Article
Effects of mutations altering SOS regulation on a nalidixic acid-inducible system for the production of heterologous proteins in Escherichia coli.
Journal of industrial microbiology - 1 Nov 1990
Keller J, Gerber R, Del Tito B J, Vannicolo D, Arcuri E J
Abstract excerpt
The major leftward early promoter of phage lambda pL, has frequently been used to drive expression of heterologous genes in Escherichia coli. pL is typically maintained fully repressed by the lambda cl protein. When induction of heterologous protein synthesis is desired, one of several potential mechanisms of destroying cl function is employed and the expression of the foreign gene commences. One method of...
Topics
- Animals
- Antigens, Protozoan
- Escherichia coli
- Fermentation
- Mutation
- Nalidixic Acid
- Plasmodium falciparum
- Protozoan Proteins
- Recombinant Fusion Proteins
- SOS Response, Genetics
