Article
Polymorphism analysis of the prion gene in BSE-affected and unaffected cattle.
Animal genetics - 1 Oct 1994
Neibergs H L, Ryan A M, Womack J E, Spooner R L, Williams J L
Abstract excerpt
Polymerase chain reaction (PCR) primers designed to amplify the octapeptide repeat region of the bovine prion gene were used to test the association of genotypes with bovine spongiform encephalitis (BSE) in 56 BSE-affected and 177 unaffected animals. Three alleles (A,B,C) were detected as single-strand conformation polymorphisms (SSCPs) and two alleles (1,2--representing six or five copies of the octapeptide...
Topics
- Alleles
- Animals
- Base Sequence
- Cattle
- DNA
- DNA Primers
- Encephalopathy, Bovine Spongiform
- Genotype
- Lymphocytes
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic