Article
In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion.
Hybridoma - 1 Aug 1994
Kobrin B J, Schiff C, Zivion D, Scharff M D, Spira G
Abstract excerpt
We have shown that one of the alleles in a hybridoma was nonproductive because the sequence in the N region of the heavy chain caused it to be out of frame and to terminate prematurely. This allele became productive, and the gamma 1 heavy chain that it encoded was secreted when an adenosine was i...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- Enzyme-Linked Immunosorbent Assay
- Gene Rearrangement, B-Lymphocyte
- Hybridomas
- Immunoglobulin G
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Immunoglobulin kappa-Chains
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed