Article
Low cytoplasmic mRNA levels of immunoglobulin kappa light chain genes containing nonsense codons correlate with inefficient splicing.
The EMBO journal - 3 Oct 1994
Lozano F, Maertzdorf B, Pannell R, Milstein C
Abstract excerpt
We have previously reported down-regulation of mRNA expression of some of the kappa light chain transgenes in a hybridoma derived from a secondary immune response. Of the five heavily mutated transgene copies present in that hybridoma, three included premature stop codons and were poorly represented at the mRNA level. Here we show that the nonsense mutations are the cause of the low mRNA levels. While we found no...
Topics
- Animals
- Base Sequence
- Codon, Nonsense
- Cytoplasm
- Down-Regulation
- Genes, Immunoglobulin
- Hybridomas
- Immunoglobulin kappa-Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data