Article
A well-differentiated B-cell line is permissive for somatic mutation of a transfected immunoglobulin heavy-chain gene.
Proceedings of the National Academy of Sciences of the United States of America - 28 Mar 1995
Zhu M, Rabinowitz J L, Green N S, Kobrin B J, Scharff M D
Abstract excerpt
pSV2neo plasmids containing an IgM heavy-chain gene with nonsense mutations in either the variable (V) or the constant (C) region were transfected into four differentiated mouse plasma cell lines: S107 and the NSO fusion partner (myeloma cell lines) and 2C3 and 36.65 (hybridoma cell lines). The frequencies of reversion of the nonsense mutations in multiple independent transfectants were determined with the spot...
Topics
- Amino Acid Sequence
- Animals
- B-Lymphocytes
- Base Sequence
- Cell Line
- Enzyme-Linked Immunosorbent Assay
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Constant Regions
- Immunoglobulin Heavy Chains