Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing.

doi:10.1111/j.1365-2052.1995.tb02693.x

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Article

Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing.

Animal genetics - 1 Dec 1995

Sitte K, East I J, Lavin M F, Jazwinska E C

Abstract excerpt

A sample of 52 mixed-breed dairy cattle (Holstein Friesian and Jersey) and 51 beef cattle (Hereford) from south-east Queensland was studied. The second exon of BoLA-DRB3 was amplified by polymerase chain reaction (PCR), and polymorphisms were detected by heteroduplex analysis. A large number of d...

Topics

Alleles
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
Exons
Genes, MHC Class I
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Sequence Alignment
Sequence Analysis, DNA

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