Article
Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing.
Animal genetics - 1 Dec 1995
Sitte K, East I J, Lavin M F, Jazwinska E C
Abstract excerpt
A sample of 52 mixed-breed dairy cattle (Holstein Friesian and Jersey) and 51 beef cattle (Hereford) from south-east Queensland was studied. The second exon of BoLA-DRB3 was amplified by polymerase chain reaction (PCR), and polymorphisms were detected by heteroduplex analysis. A large number of different heteroduplex patterns indicated extensive sequence polymorphism. Direct sequencing of PCR products from 17...
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