Article
'Fingerprinting' of HLA-DQA by polymerase chain reaction and heteroduplex analysis.
Molecular and cellular probes - 1 Apr 1996
Martinelli G, Trabetti E, Farabegoli P, Buzzi M, Zaccaria A, Testoni N, Amabile M, Casartelli A, de Vivo A, Pignatti P F, Tura S
Abstract excerpt
We have developed a rapid, non-radioisotopic PCR fingerprinting technique for analysis of the HLA-Class II DQA gene second exon polymorphism, and have applied it to DNA samples from 210 healthy individuals. The technique is based on the formation of specific patterns (fingerprints) of homoduplexes or heteroduplexes between in vitro amplified DNA sequences. After electrophoresis on non-denaturing polyacrylamide...
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