Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro.

doi:10.1002/j.1460-2075.1995.tb07226.x

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Article

Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro.

The EMBO journal - 15 May 1995

Hayashi T, Yamasaki S, Nauenburg S, Binz T, Niemann H

Abstract excerpt

The interaction of the presynaptic membrane proteins SNAP-25 and syntaxin with the synaptic vesicle protein synaptobrevin (VAMP) plays a key role in the regulated exocytosis of neurotransmitters. Clostridial neurotoxins, which proteolyze these polypeptides, are potent inhibitors of neurotransmission. The cytoplasmic domains of the three membrane proteins join into a tight SDS-resistant complex (Hayashi et al.,...

Topics

Bacterial Toxins
Base Sequence
Carrier Proteins
Macromolecular Substances
Membrane Fusion
Membrane Proteins
Models, Biological
Molecular Sequence Data
Munc18 Proteins
Mutation
Nerve Tissue Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Neurotoxins

Protein Binding

Protein Conformation

Protein Structure, Secondary

Qa-SNARE Proteins

R-SNARE Proteins

Recombinant Proteins

Source

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Identifiers

PMID: 7774590
DOI: 10.1002/j.1460-2075.1995.tb07226.x
PMCID: PMC398339

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7774590-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro.PMID 7774590Read ↗

Rapid and selective binding to the synaptic SNARE complex suggests a modulatory role of complexins in neuroexocytosis.Semantic proximity Read ↗

A conserved membrane attachment site in alpha-SNAP facilitates N-ethylmaleimide-sensitive factor (NSF)-driven SNARE complex disassembly.Semantic proximity Read ↗

NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin.Semantic proximity Read ↗

Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly.Semantic proximity Read ↗

A discontinuous SNAP-25 C-terminal coil supports exocytosis.Semantic proximity Read ↗

Gβγ inhibits exocytosis via interaction with critical residues on soluble N-ethylmaleimide-sensitive factor attachment protein-25.Semantic proximity Read ↗

Regulation of neuronal SNARE assembly by the membrane.Semantic proximity Read ↗

SNARE-complex disassembly by NSF follows synaptic-vesicle fusion.Semantic proximity Read ↗

SNARE complex formation is triggered by Ca2+ and drives membrane fusion.Semantic proximity Read ↗

SNARE function analyzed in synaptobrevin/VAMP knockout mice.Semantic proximity Read ↗

Synaptobrevin/vesicle-associated membrane protein (VAMP) of Aplysia californica: structure and proteolysis by tetanus toxin and botulinal neurotoxins type D and F.Semantic proximity Read ↗

Complexin is able to bind to SNARE core complexes in different assembled states with distinct affinity.Semantic proximity Read ↗

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