Article
NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin.
eLife - 9 Jul 2018
Choi Ucheor B, Zhao Minglei, White K Ian, Pfuetzner Richard A, Esquivies Luis, Zhou Qiangjun, Brunger Axel T
Abstract excerpt
SNARE complex disassembly by the ATPase NSF is essential for neurotransmitter release and other membrane trafficking processes. We developed a single-molecule FRET assay to monitor repeated rounds of NSF-mediated disassembly and reassembly of individual SNARE complexes. For ternary neuronal SNARE complexes, disassembly proceeds in a single step within 100 msec. We observed short- (<0.32 s) and long-lived (≥0.32...
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