Article
Expression of a dominant negative inhibitor of intercellular communication in the early Xenopus embryo causes delamination and extrusion of cells.
Development (Cambridge, England) - 1 Feb 1995
Paul D L, Yu K, Bruzzone R, Gimlich R L, Goodenough D A
Abstract excerpt
A chimeric construct, termed 3243H7, composed of fused portions of the rat gap junction proteins connexin32 (Cx32) and connexin43 (Cx43) has been shown to have selective dominant inhibitory activity when tested in the Xenopus oocyte pair system. Co-injection of mRNA coding for 3243H7 together wit...
Topics
- Animals
- Base Sequence
- Blastocyst
- Blotting, Western
- Cell Adhesion
- Chimera
- Connexin 43
- Connexins
- DNA Primers
- Embryonic Induction
- Female
- Gap Junctions
- Gene Expression
- Genetic Techniques
- Microinjections
- Molecular Sequence Data
- Oocytes
- Phenotype