Article
Electrospray mass spectrometry characterization of post-translational modifications of barley alpha-amylase 1 produced in yeast.
Bio/technology (Nature Publishing Company) - 1 Oct 1993
Søgaard M, Andersen J S, Roepstorff P, Svensson B
Abstract excerpt
We have used electrospray mass spectrometry (ESMS) in combination with protein chemistry and genetics to delineate post-translational modifications in yeast of barley alpha-amylase 1 (AMY1), a 45 kD enzyme crucial for production of malt, an important starting material in the manufacture of beer and whisky. In addition to signal peptide processing these modifications are: (1) removal of C-terminal Arg-Ser by...
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