Article
Electrospray mass spectrometry characterization of post-translational modifications of barley alpha-amylase 1 produced in yeast.
Bio/technology (Nature Publishing Company) - 1 Oct 1993
Søgaard M, Andersen J S, Roepstorff P, Svensson B
Abstract excerpt
We have used electrospray mass spectrometry (ESMS) in combination with protein chemistry and genetics to delineate post-translational modifications in yeast of barley alpha-amylase 1 (AMY1), a 45 kD enzyme crucial for production of malt, an important starting material in the manufacture of beer a...
Topics
- Base Sequence
- Blotting, Western
- Glutathione
- Hordeum
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Folding
- Protein Processing, Post-Translational
- Recombinant Proteins
- Saccharomyces cerevisiae
- alpha-Amylases