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Article

A dual affinity tag on the 64-kDa Nlt1p subunit allows the rapid characterization of mutant yeast oligosaccharyl transferase complexes.

Archives of biochemistry and biophysics - 1 Feb 1997

Pathak R, Imperiali B

Abstract excerpt

Oligosaccharyl transferase catalyzes the glycosylation of selected asparagine residues of nascent polypeptide chains as they are translocated into the lumen of the endoplasmic reticulum. To date, this enzyme has been purified from a number of eukaryotic organisms. Purification of transferase activity has yielded polypeptide complexes of three to six subunits depending on the source organism. Here we present the...

Topics

Affinity Labels
Amino Acid Sequence
Genes, Fungal
Hexosyltransferases
Membrane Proteins
Molecular Sequence Data
Molecular Weight
Mutation
Protein Conformation
Recombinant Fusion Proteins
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transferases

Source

Identifiers

PMID: 9015380
DOI: 10.1006/abbi.1996.9812

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