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Article

Involvement of individual subsites and secondary substrate binding sites in multiple attack on amylose by barley alpha-amylase.

Biochemistry - 15 Feb 2005

Kramhøft Birte, Bak-Jensen Kristian Sass, Mori Haruhide, Juge Nathalie, Nøhr Jane, Svensson Birte

Abstract excerpt

Barley alpha-amylase 1 (AMY1) hydrolyzed amylose with a degree of multiple attack (DMA) of 1.9; that is, on average, 2.9 glycoside bonds are cleaved per productive enzyme-substrate encounter. Six AMY1 mutants, spanning the substrate binding cleft from subsites -6 to +4, and a fusion protein, AMY1-SBD, of AMY1 and the starch binding domain (SBD) of Aspergillus niger glucoamylase were also analyzed. DMA of the...

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PMID: 15697208
DOI: 10.1021/bi048100v

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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