Article
Disulfide oxidoreductase activity of Shigella flexneri is required for release of Ipa proteins and invasion of epithelial cells.
Proceedings of the National Academy of Sciences of the United States of America - 23 May 1995
Watarai M, Tobe T, Yoshikawa M, Sasakawa C
Abstract excerpt
Secretion of IpaB, IpaC, and IpaD proteins of Shigella flexneri, essential for the invasion of epithelial cells, requires a number of proteins encoded by the spa and mxi loci on the large plasmid. Introduction of dsbA::Tn5 into S.flexneri from Escherichia coli K-12 reduced invasiveness, which resulted from a decrease in the capacity to release IpaB, IpaC, and IpaD proteins into the external medium. Examination of...
Topics
- Animals
- Antigens, Bacterial
- Apoptosis
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Cell Line
- Cysteine
- Disulfides
- Epithelium
- Genes, Bacterial