Article
Two periplasmic disulfide oxidoreductases, DsbA and SrgA, target outer membrane protein SpiA, a component of the Salmonella pathogenicity island 2 type III secretion system.
The Journal of biological chemistry - 13 Aug 2004
Miki Tsuyoshi, Okada Nobuhiko, Danbara Hirofumi
Abstract excerpt
The formation of disulfide is essential for the folding, activity, and stability of many proteins secreted by Gram-negative bacteria. The disulfide oxidoreductase, DsbA, introduces disulfide bonds into proteins exported from the cytoplasm to periplasm. In pathogenic bacteria, DsbA is required to process virulence determinants for their folding and assembly. In this study, we examined the role of the Dsb enzymes...
Topics
- Animals
- Bacterial Proteins
- Blotting, Western
- Cell Division
- DNA Primers
- Disulfides
- Electrophoresis, Polyacrylamide Gel
- Female
- Genomic Islands
- Gentamicins
- HeLa Cells