Article
Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR.
Journal of clinical microbiology - 1 Mar 1995
Whelen A C, Felmlee T A, Hunt J M, Williams D L, Roberts G D, Stockman L, Persing D H
Abstract excerpt
Recent analysis of the gene encoding the beta subunit of Mycobacterium tuberculosis RNA polymerase (rpoB) has demonstrated a small region that harbors the mutations most frequently associated with rifampin resistance. Earlier reports have described a high degree of sequence conservation of rpoB among mycobacteria other than M. tuberculosis and other GC-rich bacteria that can lead to false-positive amplification...
Topics
- Amino Acid Sequence
- Base Sequence
- Drug Resistance, Microbial
- False Positive Reactions
- Genes, Bacterial
- Genotype
- Humans
- Molecular Sequence Data
- Mycobacterium tuberculosis
- Point Mutation