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Article

Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR.

Journal of clinical microbiology - 1 Mar 1995

Whelen A C, Felmlee T A, Hunt J M, Williams D L, Roberts G D, Stockman L, Persing D H

Abstract excerpt

Recent analysis of the gene encoding the beta subunit of Mycobacterium tuberculosis RNA polymerase (rpoB) has demonstrated a small region that harbors the mutations most frequently associated with rifampin resistance. Earlier reports have described a high degree of sequence conservation of rpoB among mycobacteria other than M. tuberculosis and other GC-rich bacteria that can lead to false-positive amplification...

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Identifiers

PMID: 7751357
DOI: 10.1128/jcm.33.3.556-561.1995
PMCID: PMC227990

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