Rapid Detection of Multidrug-Resistant Mycobacterium tube rculosis by Use of Real-Time PCR and High-Resolution Melt Analysis

doi:10.1128/jcm.00812-10

Article

Rapid Detection of Multidrug-Resistant Mycobacterium tube rculosis by Use of Real-Time PCR and High-Resolution Melt Analysis

2 Sept 2010

Abstract excerpt

The current study describes the development of a unique real-time PCR assay for the detection of mutations conferring drug resistance in Mycobacterium tuberculosis. The rifampicin resistance determinant region (RRDR) of rpoB and specific regions of katG and the inhA promoter were targeted for the detection of rifampin (RIF) and isoniazid (INH) resistance, respectively. Additionally, this assay was multiplexed to...

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