Article
Single-nucleotide polymorphism-based differentiation and drug resistance detection in Mycobacterium tuberculosis from isolates or directly from sputum.
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases - 1 Feb 2005
Arnold C, Westland L, Mowat G, Underwood A, Magee J, Gharbia S
Abstract excerpt
The rapid technique of pyrosequencing was used to examine 123 samples (in the form of DNA extracts and inactivated sputum) of Mycobacterium spp. Of 99 Mycobacterium tuberculosis samples investigated for single-nucleotide polymorphisms (SNPs), 68% of isoniazid-resistant isolates analysed had an AGC --> ACC mutation in katG at codon 315, resulting in the Ser --> Thr substitution associated previously with isoniazid...
Topics
- Base Sequence
- Drug Resistance, Bacterial
- Genotype
- Humans
- Molecular Sequence Data
- Mutation
- Mycobacterium tuberculosis
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Sputum