Combined Real-Time PCR and <i>rpoB</i> Gene Pyrosequencing for Rapid Identification of <i>Mycobacterium tuberculosis</i> and Determination of Rifampin Resistance Directly in Clinical Specimens

Abstract excerpt

Our laboratory has developed a rapid, sensitive, and specific molecular approach for detection in clinical specimens, within 48 h of receipt, of both Mycobacterium tuberculosis complex (MTBC) DNA and mutations within the 81-bp core region of the rpoB gene that are associated with rifampin (RIF) resistance. This approach, which combines an initial real-time PCR with internal inhibition assessment and a...

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