Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein.

Abstract excerpt

After exposure of cells to tumor necrosis factor (TNF), I kappa B alpha is rapidly degraded by a proteolytic activity that is required for nuclear localization and activation of transcription factor NF-kappa B. To investigate this problem, we have developed a cell-free system to study the degradation of I kappa B alpha initiated in vivo. In this in vitro system, characteristics of endogenous I kappa B alpha...

Topics

• Amino Acid Sequence
• Animals
• Cell Line
• Cell-Free System
• DNA-Binding Proteins
• HeLa Cells
• Humans
• I-kappa B Proteins
• Molecular Sequence Data
• Mutation
• NF-KappaB Inhibitor alpha