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Article

Characterization of NFkappaB activation by detection of green fluorescent protein-tagged IkappaB degradation in living cells.

The Journal of biological chemistry - 23 Jul 1999

Li X, Fang Y, Zhao X, Jiang X, Duong T, Kain S R

Abstract excerpt

Activation of the transcription factor NFkappaB requires rapid degradation of its inhibitor, IkappaBalpha. To facilitate the study of IkappaBalpha degradation, we fused IkappaBalpha protein to enhanced green fluorescent protein to construct IkappaBalpha-enhanced green fluorescent protein (IG). We demonstrated by both flow cytometry and Western blot analysis that the half-life of IG in the presence of human tumor...

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Identifiers

PMID: 10409681
DOI: 10.1074/jbc.274.30.21244

Genes

TNF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Characterization of NFkappaB activation by detection of green fluorescent protein-tagged IkappaB degradation in living cells.PMID 10409681Read ↗

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