Article
Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis.
Electrophoresis - 1 Jan 1995
Sugano K, Fukayama N, Ohkura H, Shimosato Y, Yamada Y, Inoue T, Sekiya T, Hayashi K
Abstract excerpt
Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor suppressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change--depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism...
Topics
- Base Sequence
- Buffers
- DNA Primers
- Electric Conductivity
- Electrophoresis, Polyacrylamide Gel
- Equipment Design
- Genes, p53
- Genes, ras
- Molecular Sequence Data
- Mutation
- Polymorphism, Single-Stranded Conformational