Article
Single-strand conformation polymorphism analysis to detect p53 mutations: characterization and development of controls.
Clinical chemistry - 1 Dec 1997
Welsh J A, Castrén K, Vähäkangas K H
Abstract excerpt
Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified p53 exons 4-8, where mutations frequently occur in human tumors. Easily obtainable and clearly...
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