Single-strand conformation polymorphism analysis to detect p53 mutations: characterization and development of controls.

Article

Single-strand conformation polymorphism analysis to detect p53 mutations: characterization and development of controls.

Clinical chemistry - 1 Dec 1997

Welsh J A, Castrén K, Vähäkangas K H

Abstract excerpt

Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation d...

Topics

Exons
Genes, p53
Humans
Mutation
Polymorphism, Single-Stranded Conformational
Reference Standards
Reproducibility of Results
Temperature

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