Article
Analysis of single-strand DNA conformation polymorphism by capillary electrophoresis.
Journal of chromatography. A - 26 Jan 1996
Arakawa H, Nakashiro S, Maeda M, Tsuji A
Abstract excerpt
Analysis of single-strand conformation polymorphism (SSCP) by capillary electrophoresis (CE) was developed. The conformational change of single-strand DNA is caused by a mutation in a DNA fragment. The change is detected as mobility shift in CE. The effects of acrylamide gel concentration, running temperature and fragment size amplified by the polymerase chain reaction (PCR) were studied to develop the separation...
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