Article
Detection of K-ras mutations in DNAs isolated from feces of patients with colorectal tumors by mutant-allele-specific amplification (MASA).
Oncogene - 6 Apr 1995
Hasegawa Y, Takeda S, Ichii S, Koizumi K, Maruyama M, Fujii A, Ohta H, Nakajima T, Okuda M, Baba S
Abstract excerpt
K-ras mutations are found in approximately half of all colorectal tumors examined. To explore the possibility of detecting mutated K-ras rapidly and efficiently in DNAs isolated from fecal material, we applied the mutant allele specific amplification (MASA)-PCR method to DNA from feces of patient...
Topics
- Adenocarcinoma
- Adenoma
- Adult
- Aged
- Alleles
- Base Sequence
- Colorectal Neoplasms
- DNA Primers
- DNA, Neoplasm
- Feces
- Female
- Genes, ras
- Humans
- Male
- Middle Aged
- Molecular Sequence Data
- Point Mutation
- Polymerase Chain Reaction