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Article

Thirtyfold multiplex genotyping of the p53 gene using solid phase capturable dideoxynucleotides and mass spectrometry.

Genomics - 1 May 2004

Kim Sobin, Ulz Michael E, Nguyen Tuan, Li Chi-Ming, Sato Takaaki, Tycko Benjamin, Ju Jingyue

Abstract excerpt

A mass spectrometry (MS) based multiplex genotyping method using solid phase capturable (SPC) dideoxynucleotides and single base extension (SBE), named the SPC-SBE, has been developed for mutation detection. We report here the simultaneous genotyping of 30 potential point mutation sites in exons 5, 7, and 8 of the human p53 gene in one tube using the SPC-SBE method. The 30 mutation sites, including the most...

Topics

Base Sequence
Codon
DNA Mutational Analysis
DNA Primers
Dideoxynucleosides
Genes, p53
Genetic Testing
Genotype
Humans
Nucleotides
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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Identifiers

PMID: 15081121
DOI: 10.1016/j.ygeno.2003.11.012

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