Article
Identification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Mori Hirotada, Baba Tomoya, Yokoyama Katsushi, Takeuchi Rikiya, Nomura Wataru, Makishi Kazuichi, Otsuka Yuta, Dose Hitomi, Wanner Barry L
Abstract excerpt
Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion...
Topics
- Amino Acid Sequence
- Base Sequence
- Conjugation, Genetic
- DNA Primers
- Electroporation
- Escherichia coli K12
- Gene Knockout Techniques
- Genes, Bacterial
- Genes, Essential
- Genes, Lethal
- Genes, Synthetic
- Genetic Complementation Test
- Genomics