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Identification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Mori Hirotada, Baba Tomoya, Yokoyama Katsushi, Takeuchi Rikiya, Nomura Wataru, Makishi Kazuichi, Otsuka Yuta, Dose Hitomi, Wanner Barry L

Abstract excerpt

Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion...

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PMID: 25636612
DOI: 10.1007/978-1-4939-2398-4_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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