Article
Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.
Virology - 1 Nov 1993
Dunn S J, Ward R L, McNeal M M, Cross T L, Greenberg H B
Abstract excerpt
Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 did not recognize identical epitopes. Direct sequencing of PCR products of gene 8, encoding VP7,...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antigens, Viral
- Base Sequence
- Capsid
- Capsid Proteins
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Epitopes