Article
Functional expression of human P-glycoprotein in Schizosaccharomyces pombe.
FEBS letters - 20 Sept 1993
Ueda K, Shimabuku A M, Konishi H, Fujii Y, Takebe S, Nishi K, Yoshida M, Beppu T, Komano T
Abstract excerpt
Human MDR1 cDNA was introduced into the human cultured cells KB-3-1 and Schizosaccharomyces pombe pmd1 null mutant KN3. The drug sensitivity of KB-G2 and KN3/pgp, expressing human P-glycoprotein, was examined. KB-G2 was resistant to the peptide antibiotics valinomycin and gramicidin D as well as having a typical multidrug resistance (MDR) phenotype. KN3/pgp was resistant to valinomycin and actinomycin D, but not...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Adenosine Triphosphate
- Base Sequence
- Carrier Proteins
- Cells, Cultured
- Cloning, Molecular
- DNA
- Dactinomycin
- Doxorubicin
- Drug Resistance
- Gramicidin