Article
bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamily.
Journal of bacteriology - 1 Mar 1995
Nagao K, Taguchi Y, Arioka M, Kadokura H, Takatsuki A, Yoda K, Yamasaki M
Abstract excerpt
We have isolated a Schizosaccharomyces pombe gene, bfr1+, which on a multicopy plasmid vector, pDB248', confers resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport. This gene encodes a novel protein of 1,531 amino acids with an intramolecular duplicated structure, each half containing a single ATP-binding consensus sequence and a set of six transmembrane sequences. This structural...
Topics
- ATP-Binding Cassette Transporters
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Brefeldin A
- Cloning, Molecular
- Cyclopentanes
- Drug Resistance, Microbial
- Drug Resistance, Multiple