Article
Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III.
The EMBO journal - 1 Jul 1993
Chiang C M, Ge H, Wang Z, Hoffmann A, Roeder R G
Abstract excerpt
Two multisubunit complexes containing the TATA-binding protein (TBP) were isolated from HeLa cells constitutively expressing the FLAG epitope-tagged TBP using antibody affinity and peptide elution methods. One of the complexes (f:TFIID), isolated from the P11 0.85 M KCl fraction, contains at least 13 specific TBP-associated factors (TAFs) and can mediate activator-dependent transcription by RNA polymerase II....
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