Article
Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III.
The EMBO journal - 1 Jul 1993
Chiang C M, Ge H, Wang Z, Hoffmann A, Roeder R G
Abstract excerpt
Two multisubunit complexes containing the TATA-binding protein (TBP) were isolated from HeLa cells constitutively expressing the FLAG epitope-tagged TBP using antibody affinity and peptide elution methods. One of the complexes (f:TFIID), isolated from the P11 0.85 M KCl fraction, contains at leas...
Topics
- Adenovirus E1B Proteins
- Adenovirus E4 Proteins
- Base Sequence
- Cloning, Molecular
- DNA-Binding Proteins
- Epitopes
- HIV
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Promoter Regions, Genetic
- RNA Polymerase II
- RNA Polymerase III
- TATA Box
- TATA-Box Binding Protein
- Transcription Factor TFIID