Article
Vertebrate TBP-like protein (TLP/TRF2/TLF) stimulates TATA-less terminal deoxynucleotidyl transferase promoters in a transient reporter assay, and TFIIA-binding capacity of TLP is required for this function.
Nucleic acids research - 15 Apr 2003
Ohbayashi T, Shimada M, Nakadai T, Wada T, Handa H, Tamura T
Abstract excerpt
The TBP-like protein (TLP/TRF2/TLF), which belongs to the TBP family of proteins, is present in all metazoan organisms. Although the human TLP has been reported to interfere with transcription from TATA-containing promoters, the transcription activation potential of TLP in higher animals is obscure. We previously demonstrated that artificially promoter-recruited TLP behaves like an unconventional transcriptional...
Topics
- Animals
- Binding, Competitive
- Cell Line
- Chickens
- DNA Nucleotidylexotransferase
- Gene Expression Regulation
- HeLa Cells
- Humans
- Luciferases
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- Recombinant Fusion Proteins