Article
Mechanistic Differences in Transcription Initiation at TATA-Less and TATA-Containing Promoters.
Molecular and cellular biology - 1 Jan 2018
Donczew Rafal, Hahn Steven
Abstract excerpt
A yeast in vitro system was developed that is active for transcription at both TATA-containing and TATA-less promoters. Transcription with extracts made from cells depleted of TFIID subunit Taf1 demonstrated that promoters of both classes are TFIID dependent, in agreement with recent in vivo findings. TFIID depletion can be complemented in vitro by additional recombinant TATA binding protein (TBP) at only the...
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