Article
Isolation of germinal centerlike events from human spleen RNA. Somatic hypermutation of a clonally related VH6DJH rearrangement expressed with IgM, IgG, and IgA.
The Journal of clinical investigation - 1 Apr 1993
Varade W S, Insel R A
Abstract excerpt
12 rearranged human VH6 immunoglobulin heavy chain genes arising from the same rearrangement were isolated without preselection from the RNA of a fragment of human spleen. The 12 clones were isolated from a pool of 31 unique VH6 clones arising from 18 unique rearrangements. 2 of the 12 related cl...
Topics
- B-Lymphocytes
- Base Sequence
- Clone Cells
- Gene Rearrangement
- Genes, Immunoglobulin
- Genes, Switch
- Humans
- Immunoglobulin A
- Immunoglobulin G
- Immunoglobulin Isotypes
- Immunoglobulin M
- Immunologic Memory
- Molecular Sequence Data
- Mutation
- RNA
- Spleen