Article
Use of the most JH-proximal human Ig H chain V region gene, VH6, in the expressed immune repertoire.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jun 1993
Varade W S, Marin E, Kittelberger A M, Insel R A
Abstract excerpt
VH6, the most 3' human H chain V region gene, is preferentially expressed in the preimmune repertoire of the developing human fetus. To determine whether VH6 contributes to the immune repertoire, we amplified and sequenced from human spleen RNA VH6 rearrangements that were isotype-switched to IgG...
Topics
- Amino Acid Sequence
- Antibody Diversity
- Base Sequence
- Child
- Cloning, Molecular
- Female
- Gene Rearrangement
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Constant Regions
- Immunoglobulin Heavy Chains
- Immunoglobulin Joining Region
- Immunoglobulin Variable Region
- Male
- Molecular Sequence Data
- Mutation
- Peptides
- Polymerase Chain Reaction