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Article

DNA synthesis errors associated with double-strand-break repair.

Genetics - 1 Jul 1995

Strathern J N, Shafer B K, McGill C B

Abstract excerpt

Repair of a site-specific double-strand DNA break (DSB) resulted in increased reversion frequency for a nearby allele. Site-specific DSBs were introduced into the genome of Saccharomyces cerevisiae by the endonuclease encoded by the HO gene. Expression of the HO gene from a galactose-inducible promoter allowed efficient DNA cleavage at a single site in large populations of cells. To determine whether the DNA...

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Identifiers

PMID: 7672595
DOI: 10.1093/genetics/140.3.965
PMCID: PMC1206680

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