Article
Site-specific recombination determined by I-SceI, a mitochondrial group I intron-encoded endonuclease expressed in the yeast nucleus.
Genetics - 1 Mar 1992
Plessis A, Perrin A, Haber J E, Dujon B
Abstract excerpt
The Saccharomyces cerevisiae mitochondrial endonuclease I-SceI creates a double-strand break as the initiating step in the gene conversional transfer of the omega+ intron to omega- DNA. We have expressed a galactose-inducible synthetic I-SceI gene in the nucleus of yeast that also carries the I-S...
Topics
- Base Sequence
- Blotting, Southern
- Cell Nucleus
- Chromosome Deletion
- Cloning, Molecular
- DNA Damage
- DNA Repair
- DNA, Fungal
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Gene Conversion
- Gene Expression
- Introns
- Mitochondria
- Molecular Sequence Data
- Mutation
- Plasmids
- Recombination, Genetic