Article
Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation.
Molecular and cellular biology - 1 Feb 1992
Sugawara N, Haber J E
Abstract excerpt
In the yeast Saccharomyces cerevisiae, a double-strand chromosome break created by the HO endonuclease is frequently repaired in mitotically growing cells by recombination between flanking homologous regions, producing a deletion. We showed that single-stranded regions were formed on both sides of the double-strand break prior to the formation of the product. The kinetics of the single-stranded DNA were monitored...
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