Article
Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation.
Molecular and cellular biology - 1 Feb 1992
Sugawara N, Haber J E
Abstract excerpt
In the yeast Saccharomyces cerevisiae, a double-strand chromosome break created by the HO endonuclease is frequently repaired in mitotically growing cells by recombination between flanking homologous regions, producing a deletion. We showed that single-stranded regions were formed on both sides o...
Topics
- Base Sequence
- Blotting, Southern
- DNA, Fungal
- DNA, Single-Stranded
- Deoxyribonucleases, Type II Site-Specific
- Galactose
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasmids
- Recombination, Genetic
- Repetitive Sequences, Nucleic Acid
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Homology, Nucleic Acid