Article
Identification of the heterothallic mutation in HO-endonuclease of S. cerevisiae using HO/ho chimeric genes.
Current genetics - 1 Sept 1995
Meiron H, Nahon E, Raveh D
Abstract excerpt
HO-endonuclease initiates a mating-type switch in the yeast S. cerevisiae by making a double-strand cleavage in the DNA of the mating-type gene, MAT. Heterothallic strains of yeast have a stable mating type and contain a recessive ho allele. Here we report the sequence of the ho allele; ho has fo...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Deoxyribonucleases, Type II Site-Specific
- Genes, Fungal
- Genes, Mating Type, Fungal
- Molecular Sequence Data
- Point Mutation
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transformation, Genetic
- Zinc Fingers