Article
Multi-drug resistance (MDR) activity in acute leukemia determined by rhodamine 123 efflux assay.
Leukemia - 1 Sept 1995
Lamy T, Drenou B, Grulois I, Fardel O, Jacquelinet C, Goasguen J, Dauriac C, Amiot L, Bernard M, Fauchet R
Abstract excerpt
We prospectively analyzed MDR functional activity by the Rh123 efflux assay in 84 de novo acute leukemias. Thirty of the 60 AML cases (50%) showed a positive dye efflux (in more than 10% of blast cells). In 19 cases, the dye efflux was superior to 30%. Twenty-four of the 30 efflux positive cases were CD34+ and could be studied in double staining. The mean percentage of effluxing CD34+ blast cells was 54%. There...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Acute Disease
- Adolescent
- Adult
- Aged
- Drug Resistance, Multiple
- Female
- Fluorescent Dyes
- Humans
- Leukemia, Myeloid
- Male