Article
Mapping of mutations contributing to the temperature sensitivity of the Sabin 1 vaccine strain of poliovirus.
Journal of virology - 1 Sept 1995
Georgescu M M, Tardy-Panit M, Guillot S, Crainic R, Delpeyroux F
Abstract excerpt
The temperature-sensitive and attenuated phenotypes of the Sabin type 1 vaccine strain of poliovirus result from numerous point mutations which occurred in the virulent Mahoney virus parent. One of these mutations is located in a 3D polymerase (3Dpol) codon (U-6203-->C, Tyr-73-->His) and is invol...
Topics
- Animals
- Chlorocebus aethiops
- Codon
- Gene Products, pol
- Genes, pol
- Genome, Viral
- HeLa Cells
- Humans
- Mice
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Point Mutation
- Poliovirus
- Poliovirus Vaccine, Oral
- Temperature
- Transfection
- Vero Cells