Article
The 5' noncoding region of the type 2 poliovirus vaccine strain contains determinants of attenuation and temperature sensitivity.
Virology - 1 Apr 1991
Macadam A J, Pollard S R, Ferguson G, Dunn G, Skuce R, Almond J W, Minor P D
Abstract excerpt
Intratypic recombinants of P2/Sabin and P2/117, a neurovirulent vaccine revertant, have been generated in vitro using infectious cDNA clones and used to demonstrate that strong determinants of the attenuation and temperature-sensitive phenotypes of P2/Sabin reside in the 5' 492 nucleotides. In this region of the genome the viruses differ only at nucleotides 437 and 481. The ts phenotype associated with the 5'...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Viral
- Epitopes
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Poliovirus Vaccine, Oral
- Sensitivity and Specificity