Article
Correlation of RNA secondary structure and attenuation of Sabin vaccine strains of poliovirus in tissue culture.
Virology - 1 Aug 1992
Macadam A J, Ferguson G, Burlison J, Stone D, Skuce R, Almond J W, Minor P D
Abstract excerpt
Part of the 5' noncoding regions of all three Sabin vaccine strains of poliovirus contains determinants of attenuation that are shown here to influence the ability of these strains to grow at elevated temperatures in BGM cells. The predicted RNA secondary structure of this region (nt 464-542 in P3/Sabin) suggests that both phenotypes are due to perturbation of base-paired stems. Ts phenotypes of site-directed...
Topics
- Base Sequence
- Hydrogen Bonding
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Phenotype
- Poliovirus Vaccine, Oral
- RNA, Viral
- Temperature
- Vaccines, Attenuated