Article
Unbalance of L-lysine flux in Corynebacterium glutamicum and its use for the isolation of excretion-defective mutants.
Journal of bacteriology - 1 Jul 1995
Vrljic M, Kronemeyer W, Sahm H, Eggeling L
Abstract excerpt
We found that the simple addition of L-methionine to the wild type of Corynebacterium glutamicum results in excretion of the cellular building block L-lysine up to rates of 2.5 nmol/min/mg (dry weight). Biochemical analyses revealed that L-methionine represses the homoserine dehydrogenase activity and reduces the intracellular L-threonine level from 7 to less than 2 mM. Since L-lysine synthesis is regulated...
Topics
- Biological Transport
- Corynebacterium
- Glutamic Acid
- Homoserine Dehydrogenase
- Isoleucine
- Lysine
- Methionine
- Mutation