Article
Site-directed mutagenesis and feedback-resistant N-acetyl-L-glutamate kinase (NAGK) increase Corynebacterium crenatum L-arginine production.
Amino acids - 1 Jul 2012
Xu Meijuan, Rao Zhiming, Dou Wenfang, Yang Juan, Jin Jian, Xu Zhenghong
Abstract excerpt
N-acetyl-L-glutamate kinase (EC 2.7.2.8) is first committed in the specific L-arginine pathway of Corynebacterium sp. A limited increase of L-arginine production for the argB overexpression in the engineering C. creantum SYPA-CCB strain indicated that L-arginine feedback inhibition plays an influence on the L-arginine production. In this study, we have performed site-directed mutagenesis of the key enzyme (NAGK)...
Topics
- Amino Acid Sequence
- Arginine
- Corynebacterium
- Feedback, Physiological
- Gene Expression Regulation, Bacterial
- Genetic Engineering
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation