Article
Two-dimensional electrophoresis southern transfer method for detecting human genome variability using a LINE-1 sequence probe.
Analytical biochemistry - 20 May 1995
Nakashima H, Yi M, Ichikawa N, LeBlond G F, Antonarakis S E, Ts'o P O
Abstract excerpt
A "high-resolution, two-dimensional Southern transfer" method has been developed and was used to examine the distribution of a class of interspersed repeated sequences in human genomes. This method consists of two separate restriction enzyme digestions, including an in situ digestion, and two-dim...
Topics
- Base Sequence
- Blotting, Southern
- DNA Probes
- Electrophoresis, Gel, Two-Dimensional
- Female
- Genetic Variation
- Genome, Human
- Humans
- Male
- Molecular Sequence Data
- Repetitive Sequences, Nucleic Acid