Article
The use of synthetic tandem repeats to isolate new VNTR loci: cloning of a human hypermutable sequence.
Genomics - 1 Sept 1991
Vergnaud G, Mariat D, Apiou F, Aurias A, Lathrop M, Lauthier V
Abstract excerpt
Synthetic tandem repeats (STRs) of oligonucleotides have previously been shown to detect polymorphic loci in the human genome. Here, we report results from the use of three such probes to screen a human cosmid library. Nine of the 45 positive clones that were analyzed appear to contain highly polymorphic minisatellite or VNTR loci. The degree of enrichment for minisatellite sequences varied with the choice of...
Topics
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Cosmids
- DNA
- Female
- Genetic Linkage
- Genomic Library
- Humans
- Male
- Molecular Sequence Data
- Mutation