Processivity errors of gene expression in Escherichia coli.

Abstract excerpt

Not all ribosomes that initiate translation of an mRNA sequence will successfully complete it and produce a full-length protein product. By comparing the amounts of lacZ monomer and lacZ dimer protein expressed from a plasmid in a strictly controlled assay, we calculate a dimer to monomer ratio of 0.76. We interpret this to mean that ribosomes have a 76% chance of completing the synthesis of a beta-galactosidase...

Topics

• Base Sequence
• DNA-Directed RNA Polymerases
• Escherichia coli
• Gene Expression
• Lac Operon
• Molecular Sequence Data
• Mutation
• Plasmids
• Ribosomes
• beta-Galactosidase