Article
Correlation of translation efficiency with the decay of lacZ mRNA in Escherichia coli.
Journal of molecular biology - 24 Jun 1994
McCormick J R, Zengel J M, Lindahl L
Abstract excerpt
We isolated mutations in the leader of a ribosomal protein (r-protein)/lacZ fusion gene in Escherichia coli that caused the mRNA to be translated at efficiencies between < 1% and 62% of the rate of wild-type message. Using a subset of these mutants with translation efficiencies between 5% and 62%, we studied both physical and functional decay of the mRNA after rifampicin inhibition of transcription initiation....
Topics
- Base Sequence
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Lac Operon
- Molecular Sequence Data
- Mutation
- Peptide Chain Initiation, Translational
- Plasmids
- Protein Biosynthesis
- RNA, Messenger